RAF1 Knockout cell line (A549)

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RAF1 Knockout cell line (A549) Order

RAF1 Knockout cell line (A549)
Catalog#
YKO-H760
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
RAF1 Knockout cell line (A549)
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Detailed Product Information

Product category
KO Cell Lines
cell name
RAF1 Knockout cell line（A549）
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Human
Gene Name
RAF1
Gene ID
5894
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90% F12K+10% FBS
Accession
CVCL_D7Z5(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 2 (based on transcript RAF1-201)
Validation: PCR+Sanger Sequencing
RAF1 Knockout cell line (A549) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:A549		 STR Info (Cell bank)
Cell Line:A-549
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11111111
D13S317 11111111
D7S820 811811
D16S539 11121112
VWA 14141414
TH01 89.389.3
AMEL XYXY
TPOX 811811
CSF1PO 10121012
D12S391 1818
FGA 2323
D2S1338 2424
D21S11 2929
D18S51 1417
D8S1179 1314
D3S1358 1616
D6S1043 1113
PENTAE 711
D19S433 1313
PENTAD 99
D1S1656 1718.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the RAF1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the A549 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, RAF1 gene knockout RAF1 Knockout cell line（A549） will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
RAF1 Knockout cell line (A549)
RAF1 Knockout cell line (A549)
Catalog#
YKO-H760
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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